Elmada mavi küfe neden olan Penicillium expansum’a karşı bazı bor tuzlarının antifungal etkisi

Abstract

Penicillium expansum causing blue mold disease is one of the most important pathogens of pome fruit and is a necrotrophic fungus that requires wounds to infect the fruit. In the present study, the efficacy of etidot-67 and borax decahydrate against P. expansum were evaluated in both in vitro and in in vivo . In in vitro experiments, both etidot-67 and borax decahydrate strongly inhibited mycelial growth, spore germination and germ tube elongation of P. expansum . The inhibitory effects of both salts were closely correlated with their increasing concentrations. While boron salts completely inhibited the mycelial growth of P. expansum at %0.25 concentration, these salts completely inhibited spore germination and germ tube elongation of the fungus at %0.125 concentration. Additionally, it was determined that the toxicity of the salts tested were close to each other, namely EC 50 values of Etidot-67 and borax decahydrate were 0.067 and 0.071, respectively. While the minimum inhibition concentration (MIC) values of the etidot-67 and borax decahydrate were %0.25, the minimum fungicidal concentration (MFC) values of the same salts were found to be greater than 1%. In in vivo experiments, blue mold development in apple fruits treated with 3.0% concentration of etidot-67 and borax decahydrate after fungal inoculation (curative activity) was significantly reduced by %92.8 and %78.9, respectively, compared with the control treatment (P<0.05). However, at same concentration, lesion area on apple fruits treated with the salts before inoculation with the fungus (1x10 4 conidia mL -1 ) (preventive activity) reduced by %94.3 and %98.3, respectively. These results show that both boron salts can be used as a potential alternative to synthetic fungicides for the control of the postharvest disease of apple fruit caused by P. expansum .