Abstract
The effect of ellagic acid (EA), a naturally occurring polyphenolic compound, on the secretion of apolipoproteins from human hepatocytes, HepG2, was investigated. The levels of apoB and apoA-1 secreted in the cell culture medium were determined by sandwich ELISA. EA did not affect cell viability at the tested concentrations (up to 50 µM). EA suppressed the secretion of apoB and enhanced that of apoA-1 from HepG2 cells. However, cellular apoB levels were increased, suggesting that EA inhibited the trafficking of apoB during the process of secretion. In contrast, the increase in the cellular levels of apoA-1 was consistent with its secreted levels. These results indicate that EA inhibits the secretion of apoB from hepatocytes and increases the secretion of apoA-1. Both of these effects are beneficial for lipoprotein metabolism in the prevention of lifestyle-related diseases. The detailed mechanism underlying these effects of EA on lipoprotein metabolism should be elucidated in the future, but this naturally occurring polyphenolic compound might be antihyperlipidemic. Based on these results, EA is suggested as a candidate food-derived compound for the prevention of hyperlipidemia.
Highlights
Lipids play various roles in maintaining the physical functions of the body and are essential for vital activities
To clarify how ellagic acid (EA) suppressed the secretion of apolipoprotein B (apoB) from the HepG2 cells, we determined the cellular levels of apoB by western blot analysis
We investigated the effects of EA on the secretion of two apolipoproteins, apoB and apoA-1, from human HepG2 hepatoma cells
Summary
Lipids play various roles in maintaining the physical functions of the body and are essential for vital activities. Lipoproteins are primarily responsible for the transport of lipids in the blood This lipid–protein complex is comprised of a shell of apolipoproteins, phospholipids, and cholesterol around TGs and cholesterol esters. Elevated LDL cholesterol and low HDL cholesterol levels are major risk factors for the development and progression of atherosclerosis and coronary heart disease [2] These two apolipoproteins (apoB and apoA-1) secreted by the liver play an important role in lipid homeostasis. VLDLs, including apoB,inand enhance the secretion of apoA-1 be candidates for the in vivo. Dietary factors that suppress the may secretion of VLDLs,for including apoB, cluding apoB, and enhance the secretion of apoA-1 be candidates the prevention of diseases resulting from dyslipidemia. Date, several components regulating the secretion of VLDLs (apoB) from As hepatocytes have been reported.
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