Abstract
Two ELISA methods were investigated for the quantitative detection of viability and infectivity of Cryptosporidium parvum oocysts. One is to detect the sporozoites attached to the cell surface, and another is to detect the oocysts developing in host cell. It was thought that the former method, named “Fixed-cell” ELISA, could assess the viability of oocysts, and the latter method, named “Living-cell” ELISA, could assess the infectivity of oocysts. In both ELISA systems, the resulting optical density was related to the number of oocysts inoculated. Inactivation of oocyst by ultraviolet (UV) irradiation was evaluated using these two ELISA systems. One-log reduction in infectivity of oocysts was achieved by 10 mW·s·cm-2 of UV irradiation. At this UV dose, no reduction in viability determined by “Fixed-cell” ELISA was observed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
