Abstract
Strain MS-P2T was isolated from microbial mats associated with Mushroom Spring, an alkaline siliceous hot spring in Yellowstone National Park, WY, USA. The isolate grows chemoheterotrophically by oxygen-dependent respiration, and light stimulates photoheterotrophic growth under strictly oxic conditions. Strain MS-P2T synthesizes bacteriochlorophyll a and the carotenoid spirilloxanthin. However, photoautotrophic growth did not occur under oxic or anoxic conditions, suggesting that this strain should be classified as an aerobic anoxygenic phototrophic bacterium. Strain MS-P2T cells are motile, curved rods about 0.5 to 1.0 μm wide and 1.0 to 1.5 μm long. The optimum growth temperature is 45–50 °C, and the optimum pH for growth is circum-neutral (pH 7.0–7.5). Sequence analysis of the 16S rRNA gene revealed that strain MS-P2T is closely related to Elioraea species, members of the class Alphaproteobacteria, with a sequence identity of 96.58 to 98%. The genome of strain MS-P2T is a single circular DNA molecule of 3,367,643 bp with a mol% guanine-plus-cytosine content of 70.6%. Based on phylogenetic, physiological, biochemical, and genomic characteristics, we propose this bacteriochlorophyll a-containing isolate is a new species belonging to the genus Elioraea, with the suggested name Elioraea tepida. The type-strain is strain MS-P2T (= JCM33060T = ATCC TSD-174T).
Highlights
Aerobic anoxygenic phototrophic bacteria (AAPB) are bacteria that grow chemoheterotrophically by oxygen-dependent respiration, but they synthesize photosynthetic reaction centers and light-harvesting complexes containing bacteriochlorophyll (BChl) a under oxic conditions [1,2]
We describe the major characteristics of the AAPB isolate MS-P2T
Strain MS-P2T was isolated from the microbial mats associated with the main runoff channel at Mushroom Spring in the Lower Geyser Basin of Yellowstone National Park, WY, USA (GPS coordinates: Lat.: 44.5387, Long.: −110.798)
Summary
Aerobic anoxygenic phototrophic bacteria (AAPB) are bacteria that grow chemoheterotrophically by oxygen-dependent respiration, but they synthesize photosynthetic reaction centers and light-harvesting complexes containing bacteriochlorophyll (BChl) a under oxic conditions [1,2]. South Korea, is strictly aerobic, motile by swimming, and pink-pigmented with a growth temperature optimum of 28 ◦ C It has not yet been validly described, yet another thermophilic Elioraea sp., strain “Yellowstone”, was isolated from the runoff channel of Octopus Spring in YNP at 50 ◦ C, and an incomplete, draft genome sequence is available [20]. In this manuscript, we describe the major characteristics of the AAPB isolate MS-P2T from Mushroom Spring and compare its properties with those of E. tepidiphila, E. thermophila, E. rosea, and Elioraea sp. Based on its phenotypic and phylogenetic characteristics, we propose that this isolate represents a new species within the genus Elioraea with the suggested name, Elioraea tepida
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
