Abstract
The search for biologically active compounds that regulate liver function in fibrosis is an urgent medical and biological problem. A working hypothesis was tested, according to which low molecular weight biologically active compounds from Pleurotus ostreatus and Sacharamirses cerevisiae are capable of exerting immunomodulatory and antitoxic effects after intoxication of the body with ions of heavy metals, in particular copper sulfate. Elimination of the toxic effect caused by copper sulfate can also ensure the normalization of liver function in various pathologies, in particular with liver fibrosis. When determining toxicity, a study was carried out on Wistar rats, and when studying the effect of low molecular weight biologically active compounds on liver function, clinical trials were carried out on volunteers. The activity of alanine aminotransferase, aspartate aminotransferase, actonitase and glutathione peroxidase, as well as the content of bilirubin and lipid hydroperoxides were determined. It was shown that preliminary administration of biologically active compounds to rats at a dose of 0.05 mL/100 g of body weight provided the formation in some animals (up to 80%) of resistance to the toxic effect of copper sulfate (dose 2.5 mg/100 g of body weight). Such stability is associated with a shift in the balance of “prooxidants-antioxidants” towards antioxidants. The data obtained in the clinic on volunteers with liver fibrosis and hepatitis also testify in favour of the membranotropic action of biologically active compounds. Biologically active compounds provided a decrease or complete restoration of the activity of transferases (ALT and AST) in the blood serum of these patients, with the exception of one patient out of 20 examined. Our experiment has shown the relationship between the elimination of toxicity to the action of copper sulfate and the normalization of liver function in patients. The results obtained indicate that it will be promising to use a complex of low molecular weight components from P. ostreatus and S. cerevisiae as an antidote and hepatoprotective agent.
Highlights
As is well-known, the liver plays a leading role in the formation of the body’s adaptive responses to the action of various environmental factors
In the event that the animals received daily per os biologically active compounds from P. ostreatus and S. cerevisiae, which is represented by a complex of low molecular weight proteins (Fig. 2), at a dose of 0.01 mL/100 g of body weight for 6 days, and 24 hours after the last administration, they were injected with copper sulfate at a dose of 2.5 mg/100 g, as well as the control group, the mortality curve did not differ significantly from the control option (Fig. 1)
An increase in the biologically active compounds from P. ostreatus and S. cerevisiae dose to 0.4 mL/100 g of body weight did not significantly affect the resistance of experimental animals to the toxic effect of copper sulfate, as did the dose of 0.8 mL/100 g (Fig. 1b, curves IV and V)
Summary
As is well-known, the liver plays a leading role in the formation of the body’s adaptive responses to the action of various environmental factors. Pathogenetic factors of the liver are classified into biogenic (viruses, microorganisms), physical (radiation, electromagnetic fields), chemical (heavy metal ions and a wide range of hepatotropic poisons); drugs, and even some types of food and alcoholic beverages (Lynes et al, 2007; Gao & Bataller, 2011; Satapathy et al, 2015; Hirsova et al, 2016) In this regard, the liver can be considered as a metabolic mediator between environmental factors and the body, in the sense that it transforms “the actions of environmental factors into metabolic reactions of the body”, while actively rebuilding its metabolism, which is quite often accompanied by the formation of chronic pathologies – fibrosis and cirrhosis of the liver. The development of “protection” and treatment of liver diseases is an urgent biomedical and social problem since the number of people suffering from various liver pathologies in the world is even more than documented
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
