Abstract
We propose to reconstruct 3D images by combining the merits of transport of intensity and digital holography. The proposed method solves the transport-of-intensity equation by using digital holographic reconstructed images as inputs. Our simulation and experimental results show that this method can eliminate quadratic phase aberration introduced by the microscope objective in digital holographic microscopy. This proposed phase retrieval method is free of phase unwrapping process. It is thus efficient in removing quadratic phase aberration introduced by the microscope objective.
Highlights
Holography was invented in 1948 by Dennis Gabor (1948) to improve the resolution of an electron microscope. Leith and Upatnieks (1964) proposed off-axis illumination with an off-axis reference beam, eliminating the spectral overlap of the zeroth-order beam and the twin image inherent in Gabor’s in-line configuration. Schnars and Jüptner (1994) first used a CCD camera to capture a hologram and subsequently reconstruct the hologram numerically
In order to improve the transverse resolution of holographic measurements, digital holographic microscopy (DHM) was developed
To verify the effectiveness of the proposed method, a DHM system of off-axis Mach-Zehnder interferometer combined with an inverted microscopy (Olympus CKX53) has been constructed
Summary
Holography was invented in 1948 by Dennis Gabor (1948) to improve the resolution of an electron microscope. Leith and Upatnieks (1964) proposed off-axis illumination with an off-axis reference beam, eliminating the spectral overlap of the zeroth-order beam and the twin image inherent in Gabor’s in-line configuration. Schnars and Jüptner (1994) first used a CCD camera to capture a hologram and subsequently reconstruct the hologram numerically. Deep learning compensation based on convolutional neural network has shown great success (Nguyen et al, 2017) While these methods are promising, they mostly involve post processing steps for the quadratic phase removal, e.g., after holographic reconstruction. Within the method of DH-TIE, a single hologram can provide phase retrieval without phase unwrapping (Zhou et al, 2018; Yan et al, 2019; Zuo et al, 2020; Lu et al, 2021). Kelly et al (2013) compared Fresnel-based digital holography and phase retrieval from TIE All these studies provide a lot of new ideas and methods for DH-TIE applications.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
