Abstract
Using a single filtration method of 100 L (raw and settled water) or 1000 L (filtered and tap water) volumes, the elimination of human enteric viruses, coliphages and Clostridium perfringens was studied during drinking water treatment. The VIRADEL method was used for the concentration of these microorganisms on electronegative filters. Concentrates were assayed for human enteric viruses on MA-104 cells using two blind passages and our HISG-immunoperoxidase method. Coliphages were assayed by plaque assay using a nalidixic-acid resistant (selected in our laboratory) ATCC 13706 E. coli host strain by thé doulle layer agar method. Clostridium perfringens were enumerated by membrane filtration on m-CP agar (Difco). The water filtration plant studied uses full conventional treatment including ozonation and post-chlorination. The results obtained suggest that, using similar volumes of waters for the detection of these indicators, coliphages and C. perfringens are slightly more resistant than cultivable human enteric viruses and that they could thus serve as rapid indicators for the elimination of human enteric viruses in treated waters.
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