Elimination of BBTV via a systemic in vitro electrotherapy approach

Abstract

Banana bunchy top virus (BBTV) is the most destructive etiological agent limiting banana cultivation areas globally. This study attempted BBTV elimination by traditional shoot-tip culture (control) and alternative shoot-tip + electrotherapy (treated) techniques. Shoot-tip culture from Musa acuminata cv. ‘Grand Naine’ infected sources were exposed to 100 mA electric current for different time intervals (20–60 min). Virus indexing (via PCR) and genetic fidelity (by ISSR assay) from the cultures were tested, alongside the physio-biochemical parameters. Exposure of electric current for less than 50 min was ineffective for BBTV elimination. Still, a rise in the duration from 50 min or more led to eradicating the virus from some explants. Elimination of BBTV was complete from 100 % of explants exposed to 100 mA for 60 min, as confirmed by lack of BBTV detection even at six months after acclimatization. In the control treatment, the maximum efficiency of BBTV elimination was 28 % after eight subcultures. On the other hand, improved survival % was observed in the treated culture. Moreover, homogenous ISSR patterns were there between the treated and the mother plant and similar physio-biochemical activities were seen in electro-exposed cultures and healthy ones. Thus, the study reports complete BBTV-elimination from banana with international compliances, for the first time, via electrotherapy while maintaining genomic template and biochemical stability.