Abstract
Here, a magnetotactic bacterium (MTB) existing in freshwaters was acclimated and mass propagations for producing natural magnetosomes with diameter about 15 nm at ordinary ambient. It was found that the magnetosomes which are mainly composed of iron oxide and iron sulfide hold pretty well magnetic features. Furthermore, the developed magnetosomes-AFB1 antibody immunomagentic probes through chemical bonding AFB1 polyclonal antibody onto the natural magnetosomes maintain 28 fold capability on collection of AFB1 toxin in vegetable oil compared to conventional Fe3O4 magnetic nanoparticle-AFB1 antibody probes. Recovery ratio up to 93.7% from commercial vegetable oil with artificial AFB1 contamination during 15 min collection were demonstrated. Through circular dichroism (CD) spectral measurements, the mechanism of magnetosomes probe was interpreted for proposing the practical way with high-performance on controlling the aflatoxins contamination in liquid foods basing on immunomagentic separation technique.
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