Abstract
Summary Elicitation of protoplasts prepared from cultured chickpea ( Cicer arietinum L.) cells stimulated an oxidative burst (measured by luminol-dependent chemiluminescence of extracellular H 2 O 2 ) and a transient extracellulular alkalisation essentially as previously measured in cultured cell suspensions. Both responses were also induced by the protein phosphatase 2 A inhibitor cantharidin and inhibited by the protein kinase inhibitor staurosporine. Induced chemiluminescence was efficiently suppressed by exogenous application of catalase or diphenylene iodonium as well as by application of diethyldithiocarbamate. These data demonstrate that chickpea protoplasts can be elicited in the same way as suspensions of intact cells and confirm our hypothesis that the enzyme catalysing the oxidative burst in elicited chickpea cells is located in the plasma membrane and might be comparable to the mammalian NADPH oxidase.
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