Abstract

Cobalt nitrate, nickel sulphate, hydrogen peroxide, sodium nitroprusside, and culture filtrates of Pseudomonas monteili, Bacillus circularans, Trichoderma atroviridae, and Trichoderma harzianum were tested to elicit ginsenoside production in a cell suspension line of Panax quinquefolius. Abiotic elicitors preferentially increased panaxadiols whereas biotic elicitors upregulated the panaxatriol synthesis. Cobalt nitrate (50μM) increased total ginsenosides content by twofold (54.3mg/L) within 5days. It also induced the Rc synthesis that was absent in the control cultures. Elicitation with P. monteili (2.5% v/v, 5days) also supported 2.4-fold enhancement in saponin yield. Elicitation by T. atroviridae or hydrogen peroxide induced the synthesis of Rg3 and Rh2 that are absent in ginseng roots. The highest ginsenosides productivity (3.2-fold of control) was noticed in cells exposed to 1.25% v/v dose of T. atroviridae for 5days. Treating cells with T. harzianum for 15days afforded maximum synthesis and leaching (8.1mg/L) of ginsenoside Rh1.

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