Abstract
This work is to examine the rosmarinic acid (RA) accumulation and related secondary metabolism enzyme activities of Salvia miltiorrhiza Bunge (Lamiacae) hairy roots in response to a biotic elicitor, yeast extract (YE) and an abiotic elicitor, Ag +. RA accumulation as well as total phenolic content of the hairy roots was increased by both elicitors, but more significantly by YE. Preceding the elicitor-induced RA accumulation, there was a notable rise in the tyrosine aminotransferase (TAT) activity but a rapid drop in the phenylalanine ammonia-lyase (PAL) activity of roots. All elicitor effects were dependent on the elicitor dose, maximized with YE at 200 mg/L and Ag + at 15 μM. The results showed that the elicitor-induced biosynthesis of RA and phenolic compounds in the S. miltiorrhiza hairy roots was correlated with the TAT but not the PAL activity.
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