Abstract
The oval squid Sepioteuthis lessoniana is one of the most economically important squid species in Japan; however, its population structure is poorly understood due to the lack of hypervariable markers. Such information is critical for managing sustainable fisheries, as well as for ensuring the existence of wild S. lessoniana stocks. Eleven candidate microsatellite loci were isolated from a small insert genomic DNA library. Polymorphisms in these 11 loci were screened in 24 wild individuals. The number of alleles per locus was found to range from 5 to 19 alleles, and the observed heterozygosity ranged from 0.292 to 0.958. No evidence for linkage disequilibrium was detected among all the loci. The genotypic proportions conformed to Hardy-Weinberg equilibrium, except at one locus. In conclusion, these polymorphic microsatellite loci may be used to develop a genetic framework to manage S. lessoniana in the future.
Highlights
The oval squid Sepioteuthis lessoniana, a member of the Loliginidae family, is distributed over a broad geographical range throughout the Indo-Pacific region, including northern Australia, centralJapan, and eastward to the Hawaiian Islands [1]
Izuka et al [2] previously concluded that the population structure of
Twenty-four unrelated individuals were collected from Mugi, Tokushima Prefecture, and the levels of polymorphism with primer sequences and repeat motifs in the 11 microsatellite loci were evaluated (Table 1)
Summary
The oval squid Sepioteuthis lessoniana, a member of the Loliginidae family, is distributed over a broad geographical range throughout the Indo-Pacific region, including northern Australia, central. Izuka et al [2] previously concluded that the population structure of. The population structure of S. lessoniana over the primary biogeographic range in Japan remains unclear. A thorough understanding of the appropriate conservation units is a critical first step in addressing the fishery management of S. lessoniana; there is a need to identify hypervariable microsatellite loci of the species for allowing accurate evaluation of its genetic population structure. Some researchers have proposed that S. lessoniana in southwest Japan is a species complex consisting of “Aka-ika”, “Shiro-ika”, and “Kuwa-ika” types that exhibit phenotypic and genotypic differences [5]. We believe that this study will provide powerful genetic markers for studying the genetic structure of the species and for planning the management of “Shiro-ika” S. lessoniana in the future
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