Abstract

Eleven polymorphic microsatellite loci were isolated and characterized from a Chinese endemic species, Polytremis fukia Evans. The isolated loci were polymorphic, with 5–10 alleles in 21 individuals. The analyses revealed that they had different multilocus genotypes, with the expected heterozygosity ranging from 0.69 to 0.86 and observed heterozygosity ranging from 0.48 to 0.65. All loci were in Hardy-Weinberg equilibrium. Linkage disequilibrium was not detected in any pair of loci. Ten loci were amplified successfully in at least one of the eight related species. These microsatellite markers reported here are the first for P. fukia and will be used to investigate the population genetic structure and gene flow in P. fukia as well as congeneric and related species.

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