Abstract
This study examined the mechanisms underlying the effects of the vasorelaxation active substance (VAS), dimethyladenosine-5’-L-arabinose, and its partial purification fraction on nitric oxide synthase in improving erectile dysfunction with particular focus on the nitric oxide (NO)-cGMP pathways. Two rat models, 9-month-old SD rats and 11-month-old SD rats, were given VAS (40 mg/kg per day) for 4 days, The aqueous fraction of silworm male pupae extract; semi-purified VAS (100 mg/kg per day) for 10 days, respectively. The NOS activities of the following three enzymes were examined: neuronal NO synthase (nNOS), inducible NOS (iNOS), endothelial NOS (eNOS), vascular endothelial growth factor on endothelial cells (VEGF) and anti-inflammation effect of Tumor necrosis factor-α. The results showed increases in the nitric oxide synthase activities. Western blotting of the tissue homogenate showed an increase in the nNOS level in the brain and tongue, and an increase in the endothelial NO synthase (eNOS) level in penis. However, there was little association with VEGF production in HUVEC endothelial cells and no relationship with TNF-α which showed low levels.
Highlights
Of the many crude insect drugs utilized in Oriental medicine, unmated silkworm male moths are used to treat erectile dysfunction to strengthen men's vitality
This study examined the mechanisms underlying the effects of vasorelaxation active substance (VAS), a dimethyladenosine-5'-L-arabinose, and its partial purification fraction on the following: nitric oxide synthase in improving erectile dysfunction, the growth factor on endothelial cells (VEGF), and possible anti-inflammatory effect though the inhibition of of tumor necrosis factor-a (TNF-a)
Dot blot assay with neuronal NO synthase (nNOS), endothelial NO synthase (eNOS) and inducible NO synthase (NOS) (iNOS) antibody
Summary
Of the many crude insect drugs utilized in Oriental medicine, unmated silkworm male moths are used to treat erectile dysfunction to strengthen men's vitality. Dimethyladenosine-5'-L-arabinose was found to be the main component responsible for the improvement in erectile dysfunction, and may be a lead compound for the development and improvement of vasculogenic impotence drugs through phosphodiesterase inhibition and NO production in endothelial cells (Ahn et a/., 2008). The mechanism of sildenafil citrate (Viagra) and other similar drugs for the treatment of erectile dysfunc-. This study examined the mechanisms underlying the effects of vasorelaxation active substance (VAS), a dimethyladenosine-5'-L-arabinose, and its partial purification fraction on the following: nitric oxide synthase in improving erectile dysfunction, the growth factor on endothelial cells (VEGF), and possible anti-inflammatory effect though the inhibition of of TNF-a
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