Elevated temperature affects growth and hemato‐biochemical parameters, inducing morphological abnormalities of erythrocytes in Nile tilapiaOreochromis niloticus

Abstract

Temperature is considered as an important environmental factor, and the increasing water temperature resulting from global warming is a great concern. The present study was conducted to examine the effects of elevated water temperature on growth, hemato-biochemical parameters in Nile tilapia, Oreochromis niloticus acclimatized to three temperatures (31°C, 34°C and 37°C) for 60 days. Additionally, erythrocytic cellular abnormalities (ECA) and erythrocytic nuclear abnormalities (ENA) tests were assayed using peripheral erythrocytes after exposure to the three temperatures. Fish were sacrificed on days 7, 15, 30 and 60 of exposure. Growth performances viz., weight gain, % weight gain and specific growth rate (SGR) showed decreasing tendency at 34°C but significantly declined at 37°C compared to 31°C. The abundance of haemoglobin (Hb) and red blood cells (RBCs) significantly decreased in response to temperature increases, while white blood cells (WBCs) displayed the opposite response. At days 7 and 15, blood glucose levels significantly increased in response to the temperature increase, while at days 30 and 60 glucose declined. Frequencies of ECA and ENA were significantly enhanced at the highest temperature throughout the experimental period. Dissolved oxygen decreased and free CO2 increased significantly with increasing temperature throughout the study period. The present study revealed that temperatures higher than 34°C may be hazardous to O. niloticus.