Elevated Serum miR-7, miR-9, miR-122, and miR-141 Are Noninvasive Biomarkers of Acute Pancreatitis.

Pan Lu,Zhuo-Ling Li,Feng Wang,Cheng Wang,Jia-Xi Song,Jing Yan,Jun-Jun Wang,Jia Wu

doi:10.1155/2017/7293459

Pan Lu, Zhuo-Ling Li + Show 6 more

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https://doi.org/10.1155/2017/7293459

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Abstract

Background It has been reported that several microRNAs (miRNAs), such as miR-141, miR-9, and miR-122, are involved in the regulation of pancreatitis-related proteins or that their levels change in acute pancreatitis (AP) animal models. However, the serum levels, as well as the clinical diagnostic and prognostic values, of these miRNAs in AP patients remain unclear. Furthermore, as a pancreas- (islet) enriched miRNA, miR-7 was reported to be downregulated in AP patients, which requires further verification. Methods The levels of miR-7, miR-9, miR-122, and miR-141 were examined and compared using qRT-PCR among 80 severe AP patients, 80 mild AP patients, and 74 healthy controls. Results The serum levels of these four miRNAs were increased markedly in the AP patients compared with the controls, and these levels decreased significantly after effective therapy. Particularly, the level of miR-7 was higher in severe AP patients than in mild AP patients. ROC curve analysis demonstrated that four miRNAs could be used as potential biomarkers for AP. Moreover, these miRNAs showed strong positive correlations with CRP, which may be associated with inflammation. Conclusions The serum miR-7, miR-9, miR-122, and miR-141 levels were increased in AP patients. These 4 miRNAs may represent diagnostic and prognostic biomarkers for AP.

Highlights

Acute pancreatitis (AP) is a common pancreatic disease in clinical practice [1, 2]
A total of 160 patients with confirmed diagnosis of acute pancreatitis (AP) during the period of January 2016 to January 2017 and 74 control subjects are included in our analysis
Compared with the MAP patients, the levels of C reactive protein (CRP) and Glu and the APACHEII score and Ranson score were significantly increased in severe acute pancreatitis (SAP) patients (P < 0 01), while the levels of Ca2+ and HCT were decreased in SAP patients (P < 0 001)

Summary

Introduction

Acute pancreatitis (AP) is a common pancreatic disease in clinical practice [1, 2]. It has been defined as early activation of pancreatic enzymes in the pancreas, leading to autodigestion of the pancreas [3, 4]. Most AP is mild and self-limited, 10% to 20% of patients experience severe acute pancreatitis (SAP), which is a clinically lifethreatening acute abdominal disorder with a mortality rate of approximately 20% [8]. For evaluating the severity of AP, several scoring systems, such as the APACHE II (Acute Physiology and Chronic Health Evaluation II) score and Ranson score, have been used clinically. SAP patients usually experience rapid disease progression, poor prognosis, and high mortality. In view of these problems, the application of scoring systems has been limited. The serum levels, as well as the clinical diagnostic and prognostic values, of these miRNAs in AP patients remain unclear. The serum miR-7, miR-9, miR-122, and miR-141 levels were increased in AP patients These 4 miRNAs may represent diagnostic and prognostic biomarkers for AP

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