Elevated serum levels of the soluble form of gp130, the IL-6 signal transducer, in HTLV-1 infection and no involvement of alternative splicing for its generation.

Abstract

By using an ELISA, increased levels of the soluble form (sgp130) of gp130, the IL-6 signal transducer, were detected in the sera of various HTLV-1-associated conditions (HC, ATL, HAM) as compared to normal healthy individuals. Sgp130 levels seemed to be correlated with disease severity. The 94 KD of sgp130 was specifically precipitated in the sera of HTLV-1-infected patients as revealed by Western blot analysis. A reverse transcriptase (RT)-polymerase chain reaction (PCR) method was used to detect the message for transmembrane (TM) lacking gp130 in mRNA isolated from peripheral blood mononuclear cells (PBMCs) of patients infected with or without HTLV-1 and those of various hematopoietic cell lines. Two PCR products, 648 and 507 bp were observed in the PBMCs from HTLV-1-infected patients. But the 507 by PCR product was not detected in the PBMCs from normal healthy individuals and HTLV-1-positive cell lines although the 648 bp product was equally expressed. A nucleotide sequence analysis of the 507 bp fragment showed deletion of the 141 bp at the region spanning from nucleotide 1702 (G) to 1842 (T) of the 648 bp product that matched completely with a conventional gp130 molecule. This deleted region was located upstream of the transmembrane (TM) domain, but not within the TM region itself. However, no frame shift was observed. These results indicate that the generation of sgp130 may not be due to an alternative splicing mechanism.