Elevated Serum Level of DHEAS as a Hormone and IL-6 as a Proinflammatory Cytokine May Better Indicate Metabolic Syndrome in PCOS Women

Zozan Guleken ,Huri Bulut ,Pinar Yalcin Bahat ,Sezai Yilmaz ,Devrim Saribal

doi:10.7176/jmpb/71-02

Abstract

Polycystic ovarian syndrome (PCOS) is the most prevalent metabolic disorder in reproductive-age women, indeed multifactorial condition. Cytokines such as TNF-alpha and IL-6 have influence on several metabolic activities. The increase in dehydroepiandrosterone (DHEA) is thought to play an important role in IL-6-mediated pathogenetic effects in PCOS women. The aim of this study is to explore whether TNF-alpha and IL-6 are correlated hormone levels in PCOS disease. We recruited 65 PCOS patients and 45 healthy controls. The serum IL-6, TNF-alpha, levels were measured by ELISA and clinical characteristics were measured. Both serum IL-6, TNF-alpha levels (p<0.01), or the serum TSH, FSH, LH, PRL levels were significantly higher in the patients of PCOS (p<0.01). Serum IL-6 levels were correlated with DHEAS level (r=-0.4186, p<0.01). DHEAS level tended to be higher in PCOS women with a correlation between age, BMI, IL-6 level and positively correlated with FSH, LH and PRL levels (p<0.01). There was an elevation of TSH level in the PCOS group (2.4±1.5) vs. control group (1.8±1.6). However, in control samples, there was no correlation between TNF alpha and IL-6, while in the PCOS samples positive correlation, was obtained. Oral glucose tolerance test (OGTT) was higher than the control (20.38±13.2) group vs. PCOS women (128.33±33.8) but only 3.44 % of them had Type II diabetes. HOMA-IR index was higher in the PCOS group (8.26±15.06) vs. control group (2.12±0.88) too. There was a correlation between age and IL-6 level and BMI. Serum IL-6 and DHEAS levels were significantly altered in PCOS patients and determined which might be PCOS prognostic importance. Keywords; PCOS; Cytokines; TNF-alpha; IL-6; DHEAS; Metabolic syndrome DOI: 10.7176/JMPB/71-02 Publication date: June 30 th 2021

Highlights

Polycystic ovary syndrome (PCOS) is an endocrine abnormality in women of reproductive age with the prevalence ranging from 9 to 18% [1]
As a pro-inflammatory marker, we measured IL-6 level and inflammatory marker tumor necrosis factors (TNF)-alpha both in PCOS and healthy women, the results showed that IL-6 and TNF-alpha correlate in PCOS women but not in healthy women as previous studies reported both parameters may have a role in PCOS related disease [17]
PCOS is generally considered as a metabolic disorder, researchers may focus on IL-6 as an inflammatory marker and Dehydroepiandrosterone sulfate (DHEAS) as a www.iiste.org hyperandrogenic hormone and maybe the relation between these markers since that may have an important role in the management of the disease and may improve women’s health. the hormone may have an impact on the metabolic disorder

Summary

Introduction

Polycystic ovary syndrome (PCOS) is an endocrine abnormality in women of reproductive age with the prevalence ranging from 9 to 18% [1]. Cytokines soluble proteins which are small molecules are produced in response to antigens They are initially defined as mediators for regulating the adaptive immune systems [5]. Cytokines serve as chemical messengers such as tumor necrosis factors (TNF), interleukins (IL), interferons, chemokines, and growth factors [6]. They mediate in intracellular signaling pathways which are characterized by proinflammatory and anti-inflammatory effects [7]. Chronic inflammation is characterized by an increase in proinflammatory cytokines and chemokines which is associated with insulin resistance [14]. In the light of the foregoing consideration, we aimed to www.iiste.org compare circulating hormone levels by cytokines to contribute the relation between hormone concentrations and inflammation in PCOS women

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