Abstract
Expressions of gelsolin and α-actin have been investigated in a revertant cell line R1 and compared with the parental human activated Ha- ras oncogene-transformed NIH 3T3 ( EJ- NIH 3T3 ), untransformed NIH 3T3 and partially revertant R2 cells. Gelsolin mRNA expression was strongest in R1 cells, intermediate in R2 and NIH 3T3 cells, and low in EJ- NIH 3T3 cells. Southern blot analysis gave neither signs of gross rearrangements nor amplification of the gelsolin gene. α-actin mRNA expression was restored in R1 cells to the level of NIH 3T3 cells. In R2 and EJ- NIH 3T3 cell lines, no α-actin transcript was detected. High gelsolin expression and restoration of α-actin expression may be associated with the acquirement of flat morphology and ordered cell growth pattern, which imply loss of tumorigenicity of R1 cells.
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