Abstract
BackgroundPrevious studies have shown that the adaptive immunity function of T cells in disease states correlates with CD3 surface expression closely. During routine assessment of TBNK subsets in peripheral blood of pediatric patients by flow cytometry, we noticed that variable expression levels of CD3 on CD3+CD4−CD8− double-negative T (DNT) lymphocytes in different patients. The objective of this study was to assess the relationship of CD3 expression levels on DNT cells with disease severity.MethodsIn this prospective study, we investigated the frequencies of circulating CD4−CD8− DNT cell subsets with CD3low or CD3high phenotype by flow cytometry in 76 pediatric patients with pneumonia, 55 patients with severe pneumonia (SP), and 29 healthy controls (Con).ResultsThe numbers of circulating DNT cells were similar in all groups; however, the frequency of CD3low DNT cell subsets was significantly increased in patients with pneumonia (p < 0.001) and SP (p < 0.001). The elevated CD3low DNT cell frequency showed a positive correlation with the clinical severity of pneumonia. On sub-group analysis, the frequency of CD3low DNT cells was only elevated in children with pneumonia aged <5 years, while no association was observed with the causative pathogen of pneumonia.ConclusionsThese findings suggest that CD3 expression levels on DNT cell subsets of peripheral lymphocytes may be a valuable biomarker for evaluation of immune response in pediatric infectious disease. CD3low DNT cells were elevated in children with pneumonia aged <5 years, which indicates that it may be an important research target in pediatric infectious diseases.
Highlights
Infectious diseases are a predominant cause of death among children (Kollmann et al, 2017)
To explore whether the CD3 expression levels on double-negative T (DNT) cells were related to infectious disease, we analyzed the data of TBNK subsets from pediatric pneumonia or severe pneumonia patients
We analyzed the ratios of CD3low DNT cell subsets in total circulating DNT cells and found that the proportion of CD3low DNT cells in peripheral blood of patients with pneumonia and severe pneumonia was significantly elevated (p < 0.001) and showed a positive correlation with the severity of pneumonia (p < 0.01) (Fig. 1C)
Summary
Infectious diseases are a predominant cause of death among children (Kollmann et al, 2017). In this prospective study, we investigated the frequencies of circulating CD4−CD8− DNT cell subsets with CD3low or CD3high phenotype by flow cytometry in 76 pediatric patients with pneumonia, 55 patients with severe pneumonia (SP), and 29 healthy controls (Con). On sub-group analysis, the frequency of CD3low DNT cells was only elevated in children with pneumonia aged
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