Elemental diet alters macrophage function in mice

Abstract

Administration of a chemically defined liquid elemental diet (ED) induces spontaneous bacterial translocation to mesenteric lymph nodes (MLN) in animal models. The influence of this process on host immunity is unclear. This study evaluated the effects of ED on peritoneal macrophage (PMφ) antimicrobial functions. Conventional C57/BL6 mice and endotoxin-resistant C3H/HeJ mice ( n = 60) were randomized to be pair-fed either an ED or regular chow diet (RD) for 14 days. Blood, spleen, liver, and MLN were cultured for bacteria. PMφ were harvested for: percentage Candida albicans (CA) phagocytosis, percentage killing of CA, PMφ superoxide anion (O2 −) production, and TNF-dependent macrophage cytotoxicity. Enteral feeding of ED in conventional C57/BL6 mice caused significant bacterial translocation to MLN but not other organs. Significant impairment of CA killing by PMφ occurred in the ED group and was associated with reduced O2 − production. Tumor necrosis factor (TNF)-dependent cytotoxicity of PMφ was also decreased. In endotoxin-resistant C3H/HeJ mice, bacterial translocation was not observed and PMφ antifungal functions remained similar in both RD and ED groups. Thus, enteral feeding of an elemental diet downregulates host oxidative and antimicrobial mechanisms and TNF-dependent cytotoxicity in conventional mice which may be secondary to elemental diet-induced bacterial translocation.