Elemental and Phytochemical composition of Pratia begonifolia (Wall.) Lindl. by using GF-AAS, SEM-EDAX, FTIR, GC-MS and HR-LCMS.

Abstract

Pratia begonifolia (Wall.) Lindl. belongs to the Campanulaceae family, which is traditionally used for treating kidney stones. The present study investigates the elemental, functional groups and bioactive compounds of Pratia begonifolia (Wall.) Lindl. using Graphite Flame Atomic Absorption Spectrometer (GF-AAS), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDAX), Fourier Transform Infrared Spectroscopy (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS) and High-Resolution Liquid Chromatograph Mass Spectrometer (HR-LCMS). The elemental analysis using GF-AAS revealed the presence of Chromium as the highest concentration (1.46±0.001ppm). The weight percentage of 5 elements was identified by SEM/EDAX analysis, which shows that Potassium has the highest weight percentage. In FT-IR analysis, 9 functional groups of the compounds present in the plants were identified with the wavelength of the dominant peak obtained from absorption spectra. GCMS analysis of the methanol extract revealed the presence of 12 compounds, Cyclotrisiloxane, Hexaphenyl; Methyl 11,14-Octadecadienoate; Phytol; Methyl 11,14,17-Eicosatrienoate; D-fructose,1,3,6-trideoxy-3,6-epithio-; Methyl 10-methyl-undecanoate; Beta. -d-lyxofuranoside, o-nonyl-; Ascaridole epoxide; Z, Z-6,28-Heptatriactontadien-2-One; 2,4,4-Trimethyl-1-Pentanol; Beta. -D-Mannofuranoside, 1-O-(10-Undecenyl)-; Chloroacetic Acid, 2,2-Dimethylpropyl Ester; among them Cyclotrisiloxane, Hexaphenyl (4.8%) was found to have the highest percentage area. Chloroform extract revealed the presence of 17 compounds viz. Pentacosane; Eicosane, 2-Methyl-; Hexacosane; Pentatriacontane; Dotriacontane; Phytol; Z, Z-6,28-Heptatriactontadien-2-One; Dotriacontane; Squalene; Triacontane; Eicosane; Hentriacontane; Octadecane, 2,6,10,14-Tetramethyl; Tritetracontane; Octatriacontane, 1,38-Dibromo-; 1 Octadecanoic Acid, 2-Oxo-, Methyl Ester; Eicosane, 2,6,10,14,18-Pentamethyl- and Phytol and Squalene, which have anti-bacterial properties, were also present. HR-LCMS analysis revealed the presence of Hydroxysalmeterol; 2’,4’-dihydroxychalcone; Ipecac (methylpsychotrine); Rhoifolin; Genkwanin; Anabasamine; Cuscohygrine; Eicosanedioic acid; Docosanedioic acid; Oleamide; Khayanthone as a positive ion. 2-Isoprenylemodin; Quinalizarin; Luteoline; Isoeugenitol; Ensulizole; O-Desmethylquinidine; Descarboethoxyloratadine as negative ion.