Elektrophoretische trennung und eigenschaften von isoenzymen der sauren phosphatase aus dem gehirn der ratte

Abstract

Following agar gel electrophoresis of the 20 000 X g supernatant of rat brain homogenate a single band with acid phosphatase activity could be visualized by means of azo dye techniques. By adding Triton X-100 to the homogenate, a second band with enzymatic activity was demonstrable. A third isoenzyme could be identified in the presence of zinc ions. A fourth band visible only when employing lead methods has to be considered an artefact as its appearance does not depend on the presence of a substrate. The substrate specificity of the isoenzymes and the possibilities of influencing their activity were examined. By specific choice of substrates and effectors, conditions could be defined rendering possible a selective identification of the individual isoenzymes.