ELECTROTRANSFER OF CYTOKINE GENES FOR CANCER TREATMENT

Sonam Chopra,Saulius Satkauskas

doi:10.12955/cbup.v6.1291

Sonam Chopra, Saulius Satkauskas

Open Access

https://doi.org/10.12955/cbup.v6.1291

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Abstract

Gene electrotransfer, which designates the combination of gene transfer and electroporation, is a physical method for transfecting genes into cells and tissues. Many reports for the utilization of this techniques in animals confirmed that gene electrotransfer is a safe and efficient method. One of the major advantages of electrogene therapy is that it does not result in systemic toxicity. Gene electrotransfer (GET) of plasmids encoding cytokines has been shown to generate a potent anti-tumor effect. Delivery of plasmids encoding cytokines induces not only a local immune response but a systemic one as well. Cytokines can be used to stimulate host inflammatory responses and immunity to cancers. This review aims to summarize preclinically tested cytokine genes with the help of electroporation for cancer treatment.

Highlights

Gene electrotransfer, which designates the combination of gene transfer and electroporation, is a physical method for transfecting genes into cells and tissues
For cancer cell apoptosis, synchronized cellular and humoral responses are needed as shown in Figure 1.The aim of this paper is to provide an understanding of how electroporation has been used to deliver plasmid-encoded cytokines for cancer treatment
The growth of killer cells such as lymphokine activated killer cells (LAK), cytotoxic T lymphocytes, tumor infiltrating lymphocytes and natural killer cells are stimulated by IL-2

Summary

Introduction

Gene electrotransfer, which designates the combination of gene transfer and electroporation, is a physical method for transfecting genes into cells and tissues. For cancer cell apoptosis, synchronized cellular and humoral responses are needed as shown in Figure 1.The aim of this paper is to provide an understanding of how electroporation has been used to deliver plasmid-encoded cytokines for cancer treatment It provides a brief overview of preclinical studies that are either currently underway or have been completed. Electroporation is considered as an efficient technique for the delivery of genes directly to tissues of interest and results in increasing host immunity to tumor antigens. This technique avoids the involvement of nearby tissues. IL-2 is in clinical trials for the treatment of metastatic melanoma

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