Abstract
The development of a pyrophosphate (PPi) detection method has been challenging because PPi is a natural concomitant of many enzymatic reactions. The key issue of developing chemosensors for PPi is the discrimination of PPi from excess nucleoside triphosphates (NTPs). Herein, we report a new PPi detection method modulating electrostatic interactions between a negatively charged graphene oxide (GO) and a positively charged synthetic probe 1 before and after PPi recognition. The combination of probe 1 and GO resulted in excellent selectivity towards PPi even in excess adenosine triphosphate (ATP). Consequently, this sensing system was successfully applied to real-time monitoring of a DNA polymerase chain reaction (PCR).
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