Abstract
We describe a new trypsin assay on the basis of changes in fluorescence color (red-to-blue) of a hollow microcapsule. The microcapsule assay was designed based on the manipulation of intermolecular exciton migration of a conjugated polyelectrolyte in the microcapsule as follows. (1) The red-emitting, hollow microcapsule was fabricated via electrostatic self-assembly using conjugated polyelectrolytes (polyanion) and polyarginine (polycation). (2) One of the building blocks of the microcapsule, polyarginine, could be hydrolyzed by the enzymatic action of trypsin. (3) The hydrolysis resulted in destruction of the microcapsule, leading to the release of the red-emitting, conjugated polyelectrolytes into an aqueous phase. (4) Gradually, the conjugated polymer became soluble and the red-emissive color of the conjugated polyelectrolytes turned to blue, because of dominant intramolecular exciton migration in the aqueous solution. As such, trypsin could be easily sensed by the naked eye-detectable emission color change. This emission color-changeable sensing protocol can be applied to other protease assays simply by changing the corresponding polypeptide.
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