Abstract
Porous poly- D, L-lactic acid (PDLLA) electrospinning fiber membrane was prepared, and nano-hydroxyapatite (nHA) was adsorbed and wrapped into it during the unique shrinking process of the PDLLA fiber membrane to fabricate the PDLLA/nHA composite membrane scaffold for tissue engineering. Compare with the composite fibers prepared by blend electrospinning, most of nHA particles are observed to distribute on the surface of new type composite fibers, which could significantly improve the water wettability and induce the cellular adherence. FTIR analysis indicated that the PDLLA/nHA composite fibrous membrane was formed by physical adsorption. The combination was probed by scanning electron microscope, thermo-gravimetric, water contact angle and mechanical property analysis. It was proved that the nHA particles’ content and distribution, surface wettability, modulus and tensile strength of PDLLA/nHA composite fibrous membrane were influenced by the concentration of nHA dispersion and pores on the PDLLA fiber surface. The 10.6 wt % PDLLA/nHA composite fibrous membrane exhibits a more balanced tensile strength (3.28 MPa) and surface wettability (with a water contact angle of 0°) of the composite mats. Scanning electron microscope and confocal laser scanning microscopy images of chondrocyte proliferation further showed that the composite scaffold is non-toxic. The adherence and proliferation of chondrocytes on the 10.6 wt % PDLLA/nHA fibrous membrane was significantly improved, compared with PDLLA mat. The 10.6 wt % PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.
Highlights
Tissue defects arising from trauma, tumor or other diseases already lead to a large number of tissue-repair operations being performed worldwide every year, causing extensive physical and mental suffering for patients [1,2]
The morphology of porous PDLLA fibers could be affected by electrospun ambient humidity
Composite fibrous membranes were prepared from PDLLA and nHA at different wt % HA
Summary
Tissue defects arising from trauma, tumor or other diseases already lead to a large number of tissue-repair operations being performed worldwide every year, causing extensive physical and mental suffering for patients [1,2]. The tissue graft for tissue repair is transplanted from another part of the recipient’s body or by allografts. The shortage of tissue graft donors and the significant post-operative morbidity have been major problems in tissue grafting solutions [2,3]. Tissue engineering has provided a new approach for tissue repair. Once the autologous cells take hold and grow on the scaffold, the tissue engineering scaffold can be transplanted into the patient without fear. Sci. 2018, 8, 831 ; doi:10.3390/app8050831 www.mdpi.com/journal/applsci
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