Electrospun poly(L-lactide-co-caprolactone) blended with fibrinogen as an artificial tendon for achilles tendon repair

Abstract

Event Abstract Back to Event Electrospun poly(L-lactide-co-caprolactone) blended with fibrinogen as an artificial tendon for achilles tendon repair Hongbing He1, 2* and Xujun Wu2, 3* 1 Fudan University, Institute of Peripheral Vascular Surgery, China 2 P&P Biotech Co., Ltd, R&D, China 3 Shanghai Jiaotong University, School of Materials Science and Engineering, China Introduction: Achilles tendon rupture is the most common type of sports-related injury. Incomplete healing of such tendon injuries can result in marked dysfunction and disability. The gold standard clinical treatment is surgical repair using an autograft. However, autograft repair has several disadvantages including limited availability of autograft tissues, and donor site complications. Implantation of a bioresorbable prosthesis into a tendon defect may be a useful alternative method to facilitate [AP1] the repair and regeneration of tendon. Methods: The aim of the present study was to determine the effects of electrospun poly(L-lactide-co-caprolactone) blended with fibrinogen as an artificial tendon on the healing of canine Achilles tendon defects. An Achilles tendon defect (Figure 1) was created by laterally resecting 2 cm of the Achilles tendons from 24 beagle dogs that were randomly assigned to either the experimental or control group. The experimental group was repaired with the electrospun poly(L-lactide-co-caprolactone) blended with fibrinogen artificial tendon, while the control group was repaired with an autologous tendon graft. At 14, 30, 90, and 180 days after implantation, the tendons were dissected and examined grossly, and using histology and immunohistochemistry. In addition, the micromorphology, uniaxial longitudinal tensile strength, and biochemical properties were also evaluated. Results: The tendon diameters, peri-tendinous adhesion, and inflammation scores for the experimental and control groups were compared over the course of the study. The fibrillogenesis, diameter, density, and alignment of the collagen fibrils, and the hydroxyproline content, maximum load, and modulus of elasticity were significantly improved with the electrospun poly(L-lactide-co-caprolactone) blended with fibrinogen artificial tendon treatment compared with the autologous tendon treatment. Conclusion: The electrospun poly(L-lactide-co-caprolactone) blended with fibrinogen artificial tendon was biocompatible and showed appropriate biomechanical performance, suggesting that it is a suitable candidate for future clinical use. Figure 1. Achilles tendon defect. (A) Creation of a 2 cm defect, and (B) the defect after implantation of an Achilles tendon prosthesis. Keywords: Regenerative Medicine, Scaffold, Smart material, Biodegradable material Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: He H and Wu X (2016). Electrospun poly(L-lactide-co-caprolactone) blended with fibrinogen as an artificial tendon for achilles tendon repair. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01567 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Hongbing He, Fudan University, Institute of Peripheral Vascular Surgery, Shanghai, China, Email1 Dr. Xujun Wu, Shanghai Jiaotong University, School of Materials Science and Engineering, Shanghai, China, wxj@shsongli.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hongbing He Xujun Wu Google Hongbing He Xujun Wu Google Scholar Hongbing He Xujun Wu PubMed Hongbing He Xujun Wu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.