Electrospun Composite Nanofibrous Materials Based on (Poly)-Phenol-Polysaccharide Formulations for Potential Wound Treatment.

Lidija Fras Zemljič,Zdenka Peršin,Maša Knez Hrnčič,Tjaša Kraševac Glaser,Gabrijela Petek,Urban Bren,Uroš Maver

doi:10.3390/ma13112631

Lidija Fras Zemljič, Zdenka Peršin + Show 5 more

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https://doi.org/10.3390/ma13112631

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Abstract

In this paper, we focus on the preparation of electrospun composite nanofibrous materials based on (poly)-phenol-polysaccharide formulation. The prepared composite nanofibres are ideally suited as a controlled drug delivery system, especially for local treatment of different wounds, owing to their high surface and volume porosity and small fibre diameter. To evaluate the formulations, catechin and resveratrol were used as antioxidants. Both substances were embedded into chitosan particles, and further subjected to electrospinning. Formulations were characterized by determination of the particle size, encapsulation efficiency, as well as antioxidant and antimicrobial properties. The electrospinning process was optimised through fine-tuning of the electrospinning solution and the electrospinning parameters. Scanning electron microscopy was used to evaluate the (nano)fibrous structure, while the successful incorporation of bio substances was assessed by X-ray Photoelectron Spectroscopy and Fourier transform infrared spectroscopy. The bioactive properties of the formed nanofibre -mats were evaluated by measuring the antioxidative efficiency and antimicrobial properties, followed by in vitro substance release tests. The prepared materials are bioactive, have antimicrobial and antioxidative properties and at the same time allow the release of the incorporated substances, which assures a promising use in medical applications, especially in wound care.

Highlights

Wounds have been called “the silent epidemic”, as their annual incidence in the EU27 affects around 4 million patients
In order to estimate the potential applications of such technology in wound dressings, the same procedure was applied on the viscose substrate (VIS), as a representative material used in wound healing
After 15 min, it can be seen that neither chitosan solution (CS) nor chitosan nanoparticles (CSNP) liquid formulations showed the inhibition of radicals, while, after min of exposure, the inhibition increased slightly

Summary

Introduction

Wounds have been called “the silent epidemic”, as their annual incidence in the EU27 affects around 4 million patients. An ideal wound dressing should be biocompatible and biodegradable, promote the healing process, be able to swell and absorb excess exudate and have a high porosity that allows breathing. Dressings for today’s chronic wounds come in many forms [2,3,4,5,6,7], and ultrafine and nanofibres have the potential to revolutionise wound care. In order to promote wound healing, electrospun nanofibre mats (as described in research and projects) can be produced in various ways [2,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27]

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