Abstract
In this study, styrax liquidus (sweet gum balsam) extracted from Liquidambar orientalis Mill. incorporated PCL fibrous scaffolds were prepared using the electrospinning method. The effects of the styrax liquidus content on the prepared scaffolds were investigated using different physico-chemical and morphological analyses. Then, the styrax-loaded nanofibers were examined for their antioxidant activity, anti-biofilm, metal chelating, antimicrobial and DNA cleavage properties. The results obtained from these studies showed that the nanofibers exhibited effective biological activity depending on the weight ratio of the styrax liquidus. In light of the data obtained from the characterization and biological studies, a sample with high ratio of balsam was built for determining the cytocompatibility analysis in vitro. The cytotoxicity studies of the selected membrane were conducted using mouse embryonic fibroblast cells. The fibrous scaffolds lead to increase the cell number as a result of high viability. According to the results, we propose a novel biocompatible electrospun hybrid scaffold with antioxidant and antimicrobial properties that can be used as wound healing material for potential tissue engineering applications.
Highlights
Donor waiting list have more than 100,000 patients at any given time and an average of 22 people die each day while waiting for organ or tissue transportation [1]
The styrax liquidus was purchased from a local herbalist in Muğla Province, Turkey. 2,2-diphenyl-1-picrylhydrazyl (DPPH), ascorbic acid, trolox and pBR322 plasmid DNA were purchased from Sigma Aldrich, USA to be used in antimicrobial studies
These changes show that styrax is successfully dispersed in nanofiber composites
Summary
Donor waiting list have more than 100,000 patients at any given time and an average of 22 people die each day while waiting for organ or tissue transportation [1]. Synthetic or natural polymers have been used in different combinations with ceramics or essential oils of medical plants to mimic the targeted tissue in various studies [6,7,8,9]. By incorporation of these materials into the polymeric scaffolds, an ideal biomaterial can be obtained via mimicking the both physical and chemical properties of the natural extracellular matrix which is the important step of the production process [10, 11]. Tanaka et al produced chitosan loaded with dibasic calcium phosphate anhydrous for dental applications [13]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
