Electrospun 3D Fibrous Scaffolds for Chronic Wound Repair.

Huizhi Chen,Shucheng Wu,Lay Tan,Yan Peng

doi:10.3390/ma9040272

Huizhi Chen, Shucheng Wu + Show 2 more

Open Access

https://doi.org/10.3390/ma9040272

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Journal: Materials	Publication Date: Apr 6, 2016
Citations: 112	License type: CC BY 4.0

Affiliation: Nanyang Technological University, Ngee Ann Polytechnic

Abstract

Chronic wounds are difficult to heal spontaneously largely due to the corrupted extracellular matrix (ECM) where cell ingrowth is obstructed. Thus, the objective of this study was to develop a three-dimensional (3D) biodegradable scaffold mimicking native ECM to replace the missing or dysfunctional ECM, which may be an essential strategy for wound healing. The 3D fibrous scaffolds of poly(lactic acid-co-glycolic acid) (PLGA) were successfully fabricated by liquid-collecting electrospinning, with 5~20 µm interconnected pores. Surface modification with the native ECM component aims at providing biological recognition for cell growth. Human dermal fibroblasts (HDFs) successfully infiltrated into scaffolds at a depth of ~1400 µm after seven days of culturing, and showed significant progressive proliferation on scaffolds immobilized with collagen type I. In vivo models showed that chronic wounds treated with scaffolds had a faster healing rate. These results indicate that the 3D fibrous scaffolds may be a potential wound dressing for chronic wound repair.

Highlights

Chronic wounds represent one of the most significant unmet healthcare burdens in the world today and they are a main complication in diabetic patients
To check the fiber morphology and porous structure of both scaffolds fabricated by electrospinning with the liquid collector and conventional collector, scanning electron microscopy (SEM) was utilized
Cellular cellular growthgrowth and infiltration are usually limited to the the superficial of the flat, sheet-like mat by prepared byelectrospinning, traditional electrospinning, superficial layer layer of the flat, sheet-like fiber mat fiber prepared traditional presenting a presentingobstacle a significant obstacle in developing tissueusing replacement using[22]

Summary

Introduction

Chronic wounds represent one of the most significant unmet healthcare burdens in the world today and they are a main complication in diabetic patients. Current therapies for chronic wounds include growth factors administration [2], but a limited amount of growth factors may be insufficient to restore tissue homeostasis. Deficiencies in the extracellular matrix (ECM) and the accumulation of devitalized tissues are significant characteristics of non-healing wounds [5]. The introduction of a biofunctional construct to replace the missing or dysfunctional ECM may be beneficial. Such replacement should closely mimick natural ECM, providing physical and chemical cues for recruiting nearby skin cells into the wound bed. From this aspect, the mode of three-dimensional (3D) fibrous polymer scaffolds modified with natural ECM protein is of interest

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