Abstract
Yeast cells patterned by pulsed jet electrospray showed a high alcoholic fermentation rate. Multi-dimensional patterns of individual yeast cells were produced by varying the experimental parameters of the electrospray system. The electrospray process, which employed a vibrational electric field, could control patterns of viable yeast cells at a cellular resolution. This novel system for electrospraying viable cells can be applied to biological process engineering including whole cell biochip techniques and micro fermentation processes for biochemical studies.
Highlights
Yeast cells patterned by pulsed jet electrospray showed a high alcoholic fermentation rate
Microcontact printing, photolithography, dip-pen nanolithography, and inkjet printing are considered as methods for patterning biomaterials on substrates[2,3,4,5,6]
Previous studies presented that non-agglomerated cells that were electrically charged via pulsed electrical fields between a nozzle and a ground plate in the electrospray system showed patterns at a cellular resolution[18,20,21,22]
Summary
Yeast cells patterned by pulsed jet electrospray showed a high alcoholic fermentation rate. The patterns of individual yeast cells were fabricated at a cellular resolution, and the electrospray-patterned cells showed high-speed alcoholic fermentation rates. We generated line patterns of materials at a translation speed of 1 mm/s under a vibrating electrical field of 0.8 kV and at a frequency of 20 Hz. Nutrient broth media were patterned at a flow rate of 5 μL/h and incubated at 25 °C for 24 h.
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