Abstract
Our knowledge about bioactive tryptophan derivatives in the diet is rather limited. Consequently, our attention was focused on the efficient profiling, i.e. the structure specific detection, of novel tetrahydro-beta-carbolines and tryptophan glycoconjugates in food samples. Applying HPLC-MS/MS for screening and structural characterization, numerous products derived from the reaction of tryptophan with alpha-oxo acids and carbohydrates could be identified by means of neutral loss scanning. Subsequently, product ion experiments followed by the synthesis of the respective reference compounds accomplished structure elucidation of tryptophan derivatives.
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