Abstract
Frequencies of greater than 105 transformants per µg DNA were achieved in Bacteroides ruminicola F101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid pRRI4. Similar procedures gave frequences of 106 erythromycin resistant transformants per µg DNA with the shuttle plasmid pDP1 (19 kbp) in Bacteroides uniformis. Transformation of B. uniformis occurred at a far lower frequency (103µg) when pDP1 DNA was derived from E. coli rather than B. uniformis.
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