Abstract
The study of biopolymer modified electrofusion has been extended to nucleosides, nucleotides, polynucleotides, nucleic acids and their complexes with positively charged ligands (Ca 2+, La 3+, ethydiumbromid, daunomycin, poly- l-lysine, histone). Whereas the mononucleotides show different effects on the electrofusion of barley protoplasts, the behaviour of the polymers are quite similar: at low concentrations the electrofusion was enhanced remarkably and at higher concentrations (>0.05 mg ml −1) an inhibition effect was detected. Shielding the negative charge of phosphate groups the DNA complexes increase F r values to some extend. Quite strong enhancement of F r was also measured with nucleoproteins, e.g., nucleohistone. The essential process is the interaction between the polymers and the negatively charged protoplast membrane. This interaction was compared with the adsorption behavior of these polymers on the negative mercury electrode surface which can serve as a model for a `half membrane'. With these results a first system of `biopolymer modified electrofusion' is now available.
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