Abstract
The polymerization film of Congo red was prepared on the surface of carbon paste electrode by electropolymerization using cyclic voltammetric method. The higher catalytic activity was obtained for electrocatalytic oxidation of Dopamine, with drastic enhancement of the reversibility and peak current in 0.2 phosphate buffer solution of pH 7.0 at the sweep rate 100 mV/s. The variation of sweep rate and pH were investigated. The limit of detection of Dopamine was found to be 0.06 μM. The effect of interference studies was done by differential pulse voltammetric technique. In the simultaneous look at, Dopamine and Uric acid were well separated by cyclic voltammetric technique. The proposed method showed good sensitivity, selectivity, and reproducibility.
Highlights
Dopamine, which belongs to the catecholamine family of neurotransmitters, is synthesized in a sequential reaction in which tyrosine hydroxylase and amino acid decarboxylase convert tyrosine to L-dihydroxyphenylalanine (L-dopa), followed by decarboxylation of L-dopa to dopamine
All the chemicals are of analytical grade quality and were used as supplied without further purification. 25 × 10-4 M Congo red was prepared in double distilled water, 25 × 10-4 M Dopamine hydrochloride (DA) was prepared in 0.1 M Perchloric acid (HClO4), 25 × 10-4 M Uric acid (UA) was prepared in 0.1 M sodium hydroxide, and Phosphate buffer solution (PBS) of same ionic strength was prepared (0.2 M) by mixing appropriate ratio of sodium dihydrogen phosphate (NaH2PO4.H2O) disodium hydrogen phosphate (Na2HPO4)
The poly (Congo red) modified carbon paste electrode (MCPE) was prepared by placing 1 mM Congo red with 0.1 M NaOH in an electrochemical cell
Summary
Dopamine, which belongs to the catecholamine family of neurotransmitters, is synthesized in a sequential reaction in which tyrosine hydroxylase and amino acid decarboxylase convert tyrosine to L-dihydroxyphenylalanine (L-dopa), followed by decarboxylation of L-dopa to dopamine. Dopamine plays a vital role in the control of movements and has been associated with the motor symptoms experienced in patients with. Other studies show that the dopamine oxidation products can inhibit the function of specific proteins [2] and correlate formation of cysteine l-dopamine conjugates with dopamine-induced neurotoxicity. Changes in the level of dopamine in adrenal glands impact many aspects of brain circuitry. Parkinsonism is associated with a reduced level of dopamine; while schizophrenia is related to increased dopamine activity [3]. In vivo concentrations of dopamine are in the nanomolar range. Given the wide range of physiological and path physiological implications, the development of analytical assays for precise, low level, and selective measurement of dopamine are highly desirable [4]. It is essential to develop a simple and rapid quantification method for DA in routine analysis for diagnostic, neurological and pharmaceutical applications
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