Electropolymerization of aniline and (N-phenyl-o-phenylenediamine) for glucose biosensor application

Abstract

Fabrication of conductive polyaniline nanofibers (PAnNFs) and poly(aniline-co-N-phenyl-o-phenylenediamine) nanoflakes [P(An-co-PoPD)] can promote a high surface area which influences rapid ion exchange and hence improved sensitivity in biosensor applications. Here, fabrication of two conductive films of PAnNFs from aniline monomer and a mixture of aniline/N-phenyl-o-phenylenediamine (oPD) monomers through cyclic voltammetry (potentiodynamic electropolymerization) technique was performed. The electrochemical features of aniline monomer generate polyaniline nanofibers compared to aniline with addition of oPD monomer that generate nano-flakes. Electro-fabricated electrodes were characterized by SEM, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). A molecular recognition layer of glucose oxidase within chitosan (GOx:CHI) was applied to the electrode-supported polymer and produced chronoamperometry dose–responses to different glucose molar concentrations with sensitivities that were larger for P(An-co-PoPD) compared to PAnNFs electrode.