Electrophysiology and behavior feedback of diamondback moth,Plutella xylostella, to volatile secondary metabolites emitted by Chinese cabbage

Abstract

The volatiles, absorbed by Porapak Q and eluted by hexane, from Chinese cabbage,Brassica campestris chinesis (L.) Makino. var.communis Tsen et Lee, have been identified as allyl isothiocyanate, Z-3-hexenyl acetate, 3,7-dimethyl-l,3,6-octatriene, 2,5-hexanediol, Z-3-hexen-1-ol, nonanol, β-myrcene, α-pinene, E-2-hexen-1-ol, D-limonene, Z-3-hexenyl propanoate, linalool, geraniol, E-4-hexen-1-ol, Z-3-hexenyl isovalerate, α-terpinene, β-caryophyllene, 3-carene and α-caryophyllene, by their mass spectra and retention times in comparison with authentic samples. The first five chemicals are the main components. All components can elicite electroantennogram (EAG) responses of dia-mondback moth (DBM),Plutella xylostella, in which allyl isothiocyanate and C6alcohols and esters, i.e. Z-3-hexen-l-ol, E-2-hexen-1-ol, E-4-hexen-1-ol, Z-3-hexenyl isovalerate and 2,5-hexanediol elicite stronger EAG responses than other components, and EAG responses of female moths to them are slightly larger than those of male moths. However, EAG responses elicited by terpenes are smaller, and EAG responses from females to terpenes are smaller than those from males, but β-myrcene can evoke stronger EAG responses from both females and males. Volatiles from intact Chinese cabbage, allyl isothiocyanate, 2,5-hexanediol and Z-3-hexenyl isovalerate intensely cause DBM directional flying and landing in wind tunnel, in which volatiles from intact Chinese cabbage have the strongest activity, and the next is allyl isothiocyanate. Allyl isothiocyanate is more attractive to females than to males a bit. As for α-terpinene and other subordinate components, EAG and wind tunnel test make sure they have weak activity.