Electrophysiological responses of the rice striped stem borer Chilo suppressalis to volatiles of the trap plant vetiver grass (Vetiveria zizanioides L.)

Abstract

Vetiver grass (Vetiveria zizanioides L.) was previously found to effectively attract female adults of Chilo suppressalis (Walker), an important pest of rice. To determine the volatile compounds involved in this attraction, electroantennography (EAG) responses to seven synthetic volatiles released from vetiver plants were examined. Our results indicated that the responses of C. suppressalis adult antennae to the different compounds varied widely. The compounds elicited strong EAG responses in female antennae were subsequently selected for further EAG response tests, namely, caryophyllene, β-ocimene, linalool and α-pinene. EAG responses to a combination of these four compounds did not differ significantly from the individual compounds. However, pair combination tests indicated that 0.01 μg μL−1 linalool and 50 μg μL−1 α-pinene, 50 μg μL−1 caryophyllene and 0.01 μg μL−1 linalool, 0.01 μg μL−1 β-ocimene and 0.01 μg μL−1 linalool, and 0.01 μg μL−1 β-ocimene and 50 μg μL−1 caryophyllene elicited significantly greater EAG responses in 3-day female moths compared to the 1-day female. These compound combinations and the corresponding ratios are probably playing an important role in attracting female adults of C. suppressalis to the vetiver grass.