Electrophysiological properties of rostral medullary respiratory neurones in the cat: an intracellular study.

Abstract

1. We recorded the membrane potentials of sixty-three respiratory neurones in the rostral, ventral medulla of decerebrate vagotomized cats. Stable recordings were obtained in thirty-eight expiratory and twenty-five inspiratory neurones. Axonal projections were identified by antidromic invasion after electrical stimulation of the region of the dorsal respiratory group (DRG), spinal cord, and the cervical vagus, superior laryngeal and pharyngeal nerves. 2. Two types of expiratory neurones were encountered: those in which the membrane potential progressively depolarized (augmenting neurons, n = 22) and those in which the membrane potential repolarized (decrementing or post-inspiratory neurones, n = 16) during the interval between phrenic bursts. Both types were hyperpolarized during inspiration by chloride-dependent, inhibitory postsynaptic potentials (IPSPs) which decreased membrane resistance. In augmenting neurones two waves of IPSPs appeared, one early and one late in inspiration. 3. Five out of seventeen augmenting expiratory neurones tested were antidromically activated by contralateral stimulation of the spinal cord (n = 3) or the DRG (n = 2). Spinal axons were not detected in any of the sixteen decrementing expiratory neurones tested. Of thirteen expiratory neurones tested with pharyngeal nerve stimulation, one (an augmenting type) was antidromically activated. Superior laryngeal or vagal axons could not be demonstrated for any expiratory neurones. 4. Two types of inspiratory neurones were also encountered: those displaying progressive depolarization throughout inspiration (n = 5) and those which gradually repolarized after maximal depolarization at the onset of inspiration (n = 10). None of the former had identifiable spinal or medullary axons, but superior laryngeal axons were demonstrated in three and pharyngeal axons were found in three. None of the latter was antidromically activated from any of the sites stimulated. 5. Stimulation of the superior laryngeal or pharyngeal nerves evoked excitatory postsynaptic potentials (EPSPs) in all neurones except in post-inspiratory neurones. In these, stimulation of the superior laryngeal or pharyngeal nerves evoked IPSPs in five of twelve neurones tested. 6. We conclude that a spectrum of respiratory neurones lie within or ventral to the retrofacial nucleus. These neurones may control upper-airway muscles or may play a role in chemoreception.