Electrophysiological Approaches to Studying Normal and Abnormal Retinotectal Circuit Development in the Xenopus Tadpole.

Abstract

The Xenopus tadpole retinotectal projection is the main component of the amphibian visual system. It comprises the retinal ganglion cells (RGCs) in the eye, which project an axon to synapse onto tectal neurons in the optic tectum. There are many attributes of this relatively simple projection that render it uniquely well-suited for studying the functional development of neural circuits. One major experimental advantage of this circuit is that it can be genetically or pharmacologically altered and then assessed at high resolution via whole-cell electrophysiological recordings using an ex vivo isolated brain preparation. This protocol provides instructions for performing such electrophysiological investigations using the ex-vivo-isolated brain preparation. It allows one to measure many different aspects of synaptic transmission between the RGC axons and individual postsynaptic tectal neurons, including AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) to NMDA (N-methyl-d-aspartate) ratios, strength of individual RGC axons, paired pulse facilitation, and strength of individual synapses.