Abstract
1. 1. We developed an isolated spinal cell preparation from adult bullfrogs. 2. 2. The average resting membrane potential was − 60mV, and an action potential was activated by positive current injection. 3. 3. The cells retained their tetrodotoxin-sensitive Na + channels and at least two kinetically different types of K + channel. 4. 4. Under K +-free conditions, responses to GABA were blocked by bicuculline while responses to glycine, taurine or β-alanine were blocked by strychnine. 5. 5. The potency of excitatory amino acids decreased in the order: kainic acid > glutamate > NMDA. 6. 6. These studies demonstrated that the isolated cells are applicable for electrophysiological and pharmacological investigations.
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