Electrophysiological and Behavioral Responses of Plutella xylostella (Lepidoptera: Plutellidae) to Volatiles from a Non-host Plant, Geranium, Pelargonium × hortorum (Geraniaceae).

Abstract

The diamondback moth (DBM), Plutella xylostella (Lepidoptera: Plutellidae), is a notorious cruciferous vegetable pest globally. Mechanically damaged geranium (Pelargonium × hortorum) can strongly repel DBM, but specific plant bioactive compounds responsible for such effects have not been identified. The headspace volatiles from wounded geranium were analyzed using gas chromatography-electroantennographic detection and gas chromatography-mass spectrometry. The electrophysiological and behavioral responses of DBM females to these chemicals and two blends were investigated. The results showed that five components myrcene, γ-terpinene, linalool, camphor, and terpinen-4-ol in geranium plants mediated the repellence of DBM mated females. These substances significantly repelled the oviposition of females, and blend-2 (a mixture of the five components with a ratio 1:5:3:4:3) was most effective. The slow-release blend-2 had a repellent range of 0.9 m and a repellent longevity of 26 days. These five substances are promising behavioral regulators of the destructive moths and could be potential candidates for "push" components in plant-based "push-pull" strategies.