Abstract
The nonspecific carboxyl esterases and alkaline and acid phosphomonoesterases were studied in the ovary, oviduct, uterus and vagina of the intact, ovariectomized, salpingectomized or hysterectomized rat by the zymogram method. In the intact animal a single prominent alkaline phosphomonoesterase was evident in each of the reproductive organs. Its activity was greater during the proestrous and estrous periods than during the metestrous or diestrous periods. On occasion, 3 other alkaline phosphomonoesterases were observed in the uterus and vagina. Following ovariectomy, a general decrease in activity of this class of enzymes occurred throughout the genital tract. Conversely, after salpingectomy or hysterectomy, alkaline phosphomonoesterase activity increased in all organs studied. In the intact animal a minimum of 7 esterases in each of the 4 reproductive organs was demonstrated. Four acid phosphomonoesterases were also evident, of which only 2 could be repeatedly reproduced. Although variations in enzyme ac...
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