Abstract

SUMMARYPaper electrophoretic separation of pigments in beet extracts and in intact tissue was investigated. Pigments in an extract were separated into 9 distinct bands upon electrophoresis with 0.15M pyridine‐citric acid buffer, pH 4.5. A technique is presented for the re‐electrophoresis of pigments in paper section of an electrophoretogram. Using a Veronal buffer (pH 8.6) during re‐electro‐phoresis, heterogeneity of the pigments in major red and major yellow bands was displayed. Under specific conditions, beet pigments in a thin section of tissue could be resolved by electrophoresis.

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