Abstract
Lucilia sericata, Chrysomya albiceps and Musca domestica larvae have been successfully used routinely for the clinical treatment of difficult necrotic and infected wounds. Degradation by proteinases contained in larval excretory/secretory (ES) products is thought to contribute to wound debridement by removal of dead tissue. However, proteinase activity may also affect host tissue remodeling processes (Chambers et al., 2003), for hundreds of years, the beneficial effects of maggots on wound healing have been documented. Insects have to defend themselves from infection by a wide variety of potential bacteria and fungi. In addition to some of flies have beneficial effects in medicine especially, L. sericata which used in Maggot Debridement Therapy (MDT). Maggot therapy success is partly due to the ingestion and killing of living microorganisms but antibacterial activity of excretion/secretion (ES) that included the salivary gland secretions and faecal products of L. sericata has been demonstrated in vitro.
Highlights
The proteins of (ES) may be affected by antimicrobial activity and beneficial effects of maggots
(2), the obtained results displayed that the number of protein bands of marker was 7 bands with molecular weights varied from 20.35 up to >150
The 10th and 11th bands of L. sericata appeared in MWs (57.5 & 55.91 kDa.) with concentrations (2.4 & 0.3%), respectively, but disappeared in marker, the molecular weight of (52.27 kDa.) just appeared in C. albiceps and disappeared in marker with concentration (1.4%), we find the concentration (0.0%) just appeared in both L. sericata, M. domestica and disappeared in marker
Summary
The proteins of (ES) may be affected by antimicrobial activity and beneficial effects of maggots. Van der plas et al, (2008) showed that the molecular weight of protein involved in beneficial effects of maggots is believed to be contained in their excretion/secretion, antimicrobial peptides found in insects playing an important role in innate immune systems and host defense mechanisms. They have attracted much attention as a novel class of antibiotics, in particular for antibiotic-resistant pathogens, because of their action mechanism of non- selective interaction with cell surface membranes of microbes (Hancock and Rozek, 2002; Zasloff 2002; Boman 2003 and Bulet et al, 2004). The classical methods for the separation of amino acids by column chromatography
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