Electrophoretic pattern of seed proteins in &lt;i&gt;Trifolium&lt;/i&gt; L. and its taxonomic implications

A Ghareeb,N M Fawzi,N M George,S Saad

doi:10.3329/bjpt.v20i1.15461

Abstract

The taxonomic delimitations of 61 taxa of the genus Trifolium L. belonging to presently accepted five sections, namely Lotoidea, Mistyllus, Vesicaria, Chronosemium and Trifolium are evaluated, based on numerical analysis of their electrophoretic seed protein profiles. The dendrogram, resulted from the hierarchical cluster analysis of SDS-PAGE profiles of seed proteins conform, with some restrictions, to the present splitting of the genus Trifolium into the sections but not into the subsections and series.DOI: http://dx.doi.org/10.3329/bjpt.v20i1.15461Bangladesh J. Plant Taxon. 20(1): 19-26, 2013 (June)

Highlights

The genus Trifolium L. (Clover) is one of the important genera of Papilionoideae of the Leguminosae with agricultural value
The seed protein profiles of examined taxa illustrated that bands in between marker weight 116KDs and 55KDs are homogenous in comparison to bands in between 50KDs and 14KDs
The dendrogram resulted from the hierarchical cluster analysis of sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) profiles of seed proteins of 61 Trifolium taxa conform, with some restrictions, to the splitting of this genus into sections, but not with the sub-sectional arrangement under the section Lotoidea and section Trifolium considered by Zohary and Heller (1984)

Summary

Introduction

The genus Trifolium L. (Clover) is one of the important genera of Papilionoideae of the Leguminosae with agricultural value. The taxonomic delimitations of 61 taxa of Trifolium are re-assessed based on the data resulted from SDS-PAGE profiles of their seed proteins. The dendrogram resulted from the hierarchical cluster analysis of SDS-PAGE profiles of seed proteins of 61 Trifolium taxa conform, with some restrictions, to the splitting of this genus into sections, but not with the sub-sectional arrangement under the section Lotoidea and section Trifolium considered by Zohary and Heller (1984).

Results

Conclusion