Abstract
AbstractA series of experiments has been undertaken to study the phenomenon of band inversion that can occur during the separation of linear double‐stranded DNA in agarose gels under constant electric field. We found that there can be a considerable band inversion when the DNA fragments are moving as a single species. When separating mixtures of fragments, as it is usually done in routine experiments, the band inversion effect is strongly reduced.Our data support the assumption that DNA–DNA interactions can play an important role in electrophoretic separations. © 1995 John Wiley & Sons, Inc.
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