Abstract
1. 1. Blood and parotoid venom proteins of Bufo americanus americanus and Bufo woodhousei fowleri were studied using vertical block polyacrylamide gel electrophoresis. 2. 2. Plasma and venom contained no components of identical electrophoretic mobility in either species. 3. 3. Venom of both soecies contained esterases; certain esterase components appeared to be species specific. 4. 4. No lipoprotein component was found in the venom of either species. 5. 5. Venom of both species contained glycoproteins. 6. 6. Use of a non-ionic detergent improved the handling of the venom. 7. 7. Wart and parotoid venom proteins were qualitatively identical. 8. 8. Plasma transferrin and albumin components of both species were polymorphic. Single and double banded transferrin and albumin phenotypes were found in both species. 9. 9. Hemoglobin of both species was found to be single banded and to have identical electrophoretic mobility. 10. 10. No seasonal or sexual variation was apparent in venom or plasma proteins of either species.
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